Chloroform‐labile trace elements in soil via fumigation‐extraction: Steps towards the soil microbial ionome beyond C:N:P

Abstract Secondary and trace elements may be limiting soil microbial functioning, albeit demand content remain largely unknown methods for their in situ detection are limited. Thus, the objective of present study was to take first step towards method development assessment ionome, that is, elemental composition communities. Chloroform (CHCl 3 ) fumigation extraction used CHCl ‐labile secondary element concentrations soils. The suitability two extractants (NH 4 NO , CaCl 2 quantification phosphorus, sulphur, potassium, sodium, magnesium, as well selenium, iron, zinc, manganese, copper, cobalt, nickel, molybdenum, vanadium, boron, silicon, barium, arsenic, cadmium, were tested six agricultural Additionally, three extractant ratios (1:5, 1:10, 1:20) durations, 1 or h, a subset Out tested, 0.01 M found best‐suited extractant. For soil‐to‐extractant ratio 1:20 with an time h best majority soils tested. In limited number soils, we able show can successfully applied barium yield fraction. Conversion values biomass, accounting contained cell envelope components, which mostly not extractable, account adsorption colloids during yet determined larger variety To overcome some limitations approach elements, pre‐extraction provide suitable solution.